مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

Objectives: It was aimed to investigate the effects of different doses of follicle stimulating hormone (FSH) and activin A on the growth and maturation of preantral mouse follicles during the in vitro culture. Methods: Preantral follicles (90-100 urn in diameter) were harvested from 6-8 week-old Syrian mice and cultured in TCM 199 culture medium for 6 days to see the effect of FSH and Activin A. Activi(LESS THAN)0.05). The number of oocytes matured and the percentage of germinal vesicle breakdown (GVBD) was 61 and 70%, respectively as compared to the control (20 and 29%, P(LESS THAN)0.05). Follicle diameter (190 urn) and survival rate (85%) increased significantly in the presence of 100 mlU/mJ of FSH as compared to the control (P(LESS THAN)0.05). But, the administration of activin A+FSH increased the effect of both factors on follicular diameter (205 urn as compared to 100 urn in control, P(LESS THAN)O.Ol). Follicle survival, oocyte maturation and GVBD rates were 91,81 and 89%, respectively (P(LESS THAN)O.O1). Conclusion: These results have suggested that exposure to FSH and activin A before the formation of antral cavity had positive effect on follicle survival and oocyte robustness. Iran. Biomed. J. 12 (2); 85-92, 2008