چكيده لاتين :
Stromal derived factor-a (SDF-l-a) is a CXC chemokine which has been demonstrated
as a recruitment factor for leukocytes to the site of inflammation, infection, injury and
following stress. This chemokine has been shown to be expressed by liver cells and in liver
diseases. Hence, the aim of this study was to examine the expression of SDF-l by
hepatocytes in responses to the stress imposed during isolation by collagenase perfusion and
under heat shock stimulation.
In this study hepatocytes (2-5 x 106) were isolated from male Sprague Dawley rat liver
and cultured in plates that were pre-coated with collagen Type-I matrix. The western and
northern blotting analysis were employed to detect SDF-l at protein and mRNA levels in
isolated and cultured hcpatocytes in response to isolation and heat shock stresses.
The SD F-l is expressed by isolated rat hepatocytes immediately after isolation and early
culture and decreased with time. SDF-l protein was highly expressed in freshly isolated cells
and decreased by time (27h) (P(P(LESS THAN)0.05). mRNA was also expressed in freshly isolated cells
(Oh) but decreased after 24h of culture (P(P(LESS THAN)O.Ol). This results also demonstrated that
expression of SDF-l by hepatocytes was increased in response to heat shock at different
time points comparing with control (P(LESS THAN)0.01).
These results demonstrated that the isolation and heat shock stresses induced the
expression of SDF-l in hepatocytes in a time-dependent manner. Accordingly, it seems that
hepatocytes mimic the experiences that liver experience after injury in oiuo and therefore,
produce stress related agents like chemokines to overcome such a injurious condition.
