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Cloning of fusion (F) protein gene of peste des petits ruminants virus (PPRV) in secretory Pichia pastoris vector.

Lotfi M. H. نويسنده , Keyvanfar H. نويسنده , Seyfi Abad Shapouri M. R. نويسنده , Goudarzi H. نويسنده , Pourbakhsh S. A. نويسنده , Kamalzade M. نويسنده

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With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study , F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cloned into pPICZ(alpha)A a secre tory expression vector of P. pastoris for first time. The insertion was proved by both production of a 218 bp segment in Nested PCR and isolation of gene from construct by restrict ion enzyme (XbaI). Finally, It was sequenced. In conclusion, after the expression of fusion (F) gene in p. pastoris expression system , it can be used in production of recombinant vaccine against PPR disease.

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