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Mesenchymal Stem Cell Isolation from the Removed Medium of Ratיs Bone Marrow Primary Culture and their Differentiation into Skeletal Cell Lineages

Baghaban Eslaminejad Mohamadreza نويسنده , Nazarian Hamid نويسنده , Taghiyar Leila نويسنده

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In all protocols for isolation of mesenchymal stem cells (MSCs), a few days after culture initiation, the medium were discarded along with its contents of non-adherent cells and the adherent cell population kept and expanded as MSCs population. In the present study, attempt was made to expand the cells suspended in removed medium of primary culture and compare them with the adherent cell population. Materials and Methods: Four days after ratיs bone marrow culture initiation, medium of the culture was collected and its suspended cells were cultureexpanded in parallel with adherent cells till passage 3. During the culture period, the cells from either group were statistically compared with respect of the time required for cell confluency (the stage in which cells cover the entire surfaces) as an index of growth rate. At the end, the cells from both cultures were evaluated in terms of their differentiation potential. Results: The primary culture of the cells from removed medium contained large colonies of spindle-shaped cells that reached into confluency after 5.36±O.5 days, while those from the adherent population possessed small colonies reaching into confluency in 8.09±O.70 days. According to the results, at all studied passages, the cells of removed medium were significantly (p