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Access to Chondrocyte Culture, with Alginate, In Iran

Esfandiary Ebrahim نويسنده , Amirpour Noshin نويسنده , FESHARAKI MEHRAFARIN نويسنده , NASR ESFAHANI MOHAMMAD HOSSEIN نويسنده , Molavi Fariba نويسنده , Molavi Farahnaz نويسنده , Nazem Khalilollah نويسنده , Razavi Shahnaz نويسنده , Shakibaei Mehdi نويسنده

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In this study, chondrocyte culture was established for the first time in Iran, and calcium alginate was used for longer culture of chondrocyte in vitro. The study was programmed in order to be used for future human chondrocyte transplantation. The cartilage specimen obtained from 50 patients who underwent total knee and hip operations in Isfahan University of Medical Sciences. Cartilage specimens were used for monolayer as well as suspension culture in alginate beads. Approximately 12±1 millions cells were harvested from the 3יd passage. The cells were round with large euchromatic nucleus and several nucleoli and small vacuoles. The cells derived from passages 1 to 4, which were grown up then, in alginate beads, showed higher staining withalcian blue. The harvested cells in some patients were immediately and successfully used for autologus transplantation. This later work will be reported separately.

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