چكيده لاتين :
In recent years, there has been a dramaticincrease in the occurrence of waterbornedisease outbreaks
caused by the Cryptosporidium parvum, and presence of this protozoan parasite in drinking water is a significant health
problem faced by the water industry. A new strategy for detection of Cryptosporidium oocysts in water samples is
polymerase chain reactorbased techniques. In this study a nested- PCR assaywas designedfor the specific amplification
of a 199 bp DNA fragmentof the gene encodingthe heat shock protein (hsp 70) of Cryptosporidium parvum oocysts. In
order to prevent the inhibition of PCR amplification by substances contained in water samples, three DNA purification
methods includingQlAamp DNA mini kit, InstaGene Matrix, MagExtractor- Genome were compared in concentrates
of tap water samples spiked with the oocysts. After it was found that the QIAamp is only efficient purification
technique, the efficiency of QlAamp and immunomagnetic separation for nested-PCR assay of various water samples
was compared. The results show that QlAamp providea useful and rapid tool for removing of PCR inhibitors. It seems
thatQlAamppurification- nestedPCR assayis a sensitive, rapidand costeffective methodfor detection of Cryptosporidium
parvum oocysts in clean water samples with turbidity (LESS THAN) 2 nephelometric turbidity unit.
