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Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody

Ghadakchi H. نويسنده , DADRAS H. نويسنده , Pourbakhsh S. A. نويسنده , Hosseini S. M. H. نويسنده

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An indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined OIug/ml, 1:3000 and 1:100, respectively. The optical densities (OD) were compared with a commercial ELISA kit. Also, the results of ELISA were compared to hemagglutination inhibition (HI) test. The correlation coefficient of the results of both ELISAs and HI was significant (P<0.05). The developed ELISA can detect antibody against IBV and is more sensitive and suitable for screening of samples in diagnostic laboratories.

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