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Frequency of Pfcrt T76 and Pfdhfr Asn-108 Drug Resistance Mutations in falciparum Malaria in Southeastern Malaria Endemic Area of Iran

Modarres Razavi M.R نويسنده , Naddaf SR نويسنده , Bras JLe نويسنده , Raesi A نويسنده , Esmaeili Rastaghi AR نويسنده , Nateghpour M نويسنده , Assmar M نويسنده

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Background: Point mutations in the genes, Pfcrt and Pfdhfr, have been identified elsewhere as strongly associated with re¬sistance to chloroquine (CQ) and pyrimethamine, respectively. Methods: Using a restriction digestion method, we report the frequency of two important mutations, T76 and Asn108, re¬spon¬sible for conferring resistance to chloroquine and pyrimethamine, respectively, in patients residing in malarious areas of southeastern Iran. Results: Ninety eight and 84% of the patients were found to harbor chloroquine resistant (theronine-76) and pyrimethamine resis¬tant (aspargin-108) mutants, respectively. Conclusions: Despite the high frequency of T76 and Asn 108 mutations in Iranian patients, combination of CQ and pyrimethamine-sulfadoxine (SP) was shown to be quite successful with 100% adequate clinical and parasitological re¬sponses.

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