عنوان به زبان ديگر :
Kinetics of Nitric Oxide Production and MTT Reduction by HSV-1 Infected Macrophages
پديد آورندگان :
EBTEKAR M. نويسنده , YARAEE R. نويسنده , AHMADIANI A. نويسنده , SABAHI F. نويسنده
چكيده لاتين :
Background: Macrophages have important role in defense
against Herpes Simplex Virus type-I (HSY-I). The present
study was performed to determine the viability and nitric oxide
(NO) production by HSY-l infected mouse peritoneal
macrophages (HIM).
Method: The viability of macrophages was evaluated using
MTT reduction assay and the production of nitrite using
Griess method.
Results: The ability of infected macrophages to reduce Tetrazolium
(MTT) was diminished at virus to cell ratios of multiplicity
of infection (MOl) of one, three and 10; but not at
O.Oland 0.1. Induction and inhibition ofNO production by HIM
were MOl dependent. The basal NO production by these cells
was inhibited at MOl of three and ten. In contrast virus to cell
ratios of 0.0 I and O. J induced low but significant enhancement
in NO production. The inability of HIM to reduce WITT at MOl
of three was significant after 12-hrs and inhibition of NO production
was initiated between 12-20 hours after infection.
Conclusion: High doses of HSY-l seem to decrease the normal
activity of macrophages by inhibiting the production of
nitric oxide.
