عنوان به زبان ديگر :
Indication of Anti-HBc Antibody Screening and HBV-DNA Detection in Diagnosing Latent Hepatitis B Virus Infection
پديد آورندگان :
Behzad-Behbahani A. نويسنده , Mafi-Nejad A. نويسنده , Tabei S. Z. نويسنده , Lankaran K. B. نويسنده , Rashidi M. نويسنده rashidi , RASOULI A. MOHAMMADIAND M. نويسنده , POURABBAS B. نويسنده , Torab Jahromi S. A. نويسنده , Salah A. R. نويسنده
چكيده لاتين :
Background: In spite of available, and sensitive screening
assay for detection of hepatitis B virus surface antigen
(HBsAg), occasional cases of post-transfusion hepatitis B virus
infection are still observed. The aim of the present study
was to assess the prevalence of positive anti hepatitis B core
(anti-HBc) and presence of HBY-DNA in serum sample of
healthy blood donors negative for both HBsAg and anti-HCY
antibody. We evaluated whether anti-HBc could be adopted as
a screening assay for blood donation.
Material and Methods: Two thousands sera negative for both
HBsAg and anti-HCY collected from healthy blood donors
tested for presence of anti-HBc antibody. All sera positive for
anti-HBc antibody were then investigated for determination of
anti-HBc and anti-HBs titers, HbeAg and anti-HBe antibody
by enzyme immunoassay (EIA). Every sample that tested
negative for HBsAg but positive for anti-HBc alone or in
combination with other serological markers was also examined
for the presence of HBY-DNA by polymerase chain reaction
(PCR).
Results: Out of 2000 HBsAg negative blood samples, 13samples (6.55%) were positive for anti-HBc. HBY-DNA was
detected in 16 of 131(12.2%) anti-HBc positive specimens.
The liver function test results were all in normal range except
in 4 (25%) of 16 HBY-DNA positive subjects.
Conclusion: Anti-HBc antibody should be tested routinely on
blood donor volunteers, and if the sera become positive regardless
of anti-HBs titer, the blood should be discarded. Further
testing for HBY -DNA is appropriate to follow up the
blood donor patient for HBY infection.
