پديد آورندگان :
Rahimi H. R. نويسنده , Kia E. B. نويسنده , Mirhendi S. H. نويسنده , TLEBI a. نويسنده , Fasihi Harandi M. نويسنده , Jalalizand N. نويسنده , Rokni M. B. نويسنده
چكيده لاتين :
Background: Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a
high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventive
and control strategy in every endemic area. Using DNA based methods for strain /genotype characterizations of E. granu/osus
have some difficulties, especially access to an efficient and pure concentration of DNA and proper primers.
Methods:Using grinder method , a pure and high concentration DNA was extracted from 10 human hydatid cysts collected from Isfahan
(central Iran) hospitals, and processed for peR reaction. Results: Using DNASIS, the primers were designed in internal
transcribed spacer 1 (ITS I) region, following analysis of 30 E. granu/osus nucleotide sequences, extracted from gene bank.
Conclusion: Thi s new and specific E. granulosus primer which amplified DNA thoroughly can be applied for molecular
studies on echinococcosis.
