پديد آورندگان :
Rezaie S. نويسنده , Khodadadi H. نويسنده , Noorbakhsh F. نويسنده , Safari M. R. نويسنده
چكيده لاتين :
Trichophyton rubrum is an anthropophil ic fungu s cau sing up to 90% of chronic cases of dermatophytosis, Several properties
of this fungus have been investigated so far. However, a few studies were carried out in the field of molecular biolo gy of
this fungus. In the present study , we tried to identify the subunit G of its vacuolar ATPase (V-ATP ase). Pairs of 21 nt
primers were designed from highly con serv ed region s of the V-ATPase subunit G genes in other fungi . Mentioned primers
were utilized in PCR using isolated genomic DNA template as well as cytopl asmic RNA of יLrubrum and the PCR and RTPCR
fragments were then sequenced. About 469 nucleotides were sequenced which encoded a polypeptide with 119 amino
ac ids. Nucleotide sequen ce comp arison in gene data banks (NCBI, NIH) for both the DNA and its dedu ced amino acid
sequence revealed significant homology with V-ATP ase subunit G genes and proteins of other eukaryotic cells . The amino
acid sequence of the encoded protein was about 84% identic al to the sequence of V-ATPase subunit G from oth er fungi. In
summary, we have cloned the first V-ATPas e subunit G of dermatophytes and characterized it as a member of this gene
family in other eukaryotic cells.
