19086

Effect of DEHP (Di-2-Ethyl Hexyl-Phthalate) on Resumption of Meiosis and in-vitro Maturation of Mouse Oocytes and Development of Itesulting Embryos

Eimani Hossein نويسنده Saberi M , Dalman Azam نويسنده , Sepehri Hoori نويسنده , Kazemi Ashtiani Saeed نويسنده , Hassani Fatemeh نويسنده , Rezazadeh Mojtaba نويسنده , Baharvand Hossein نويسنده , Shahverdi Abdolhossein نويسنده , Omani Samani Reza نويسنده

56

61

6

Introduction: DEHP [di(2-ethylhexyl) phthalate)] is widely used in plastic industry and some reproductive toxicity has been shown with it. So, this study was designed to evaluate DEHP effects on resumption of meiosis and in vitro maturation of mouse oocytes as well as development of embryos resulted from them. Material and Methods: Mice of 4-6 weeks old were administered daily doses of 50, 100, 200 J.l1 of 2.56 J.lM DEHP solution for 12 days. Immature mouse oocytes were recovered from all experimental groups and matured in MEM-a medium containing 5% FCS with and without 7.5 IU hCG and 100 mfU rFSH. IVF was performed T6 medium. Results: Resumption of meiosis and in vitro maturation were significantly lower in all experimental groups in culture media without hormones compared to controls. Fertilization and embryo development were also significantly decreased in both culture media (with and without hormones). Conclusions: This study showed the adverse effects of DEHP on in vitro maturation and embryo development in a dose dependent manner.

1203070