چكيده لاتين :
Background: Some antigens of Ii. pylori are excreted into the
stool of infected people. These antigens can be used to detect
the infection by immunoassays such as ELISA. Our aim was
to identify these antigens by immunoblotting and affinity
chromatography techniques.
Methods: Four different antigenic preparations, namely,
whole cell sonicate (WCS), outer membrane proteins (Olvllיs) ,
cytoplasmic antigens (CAs) and cell surface-associated antigens
(CSAAs) were obtained from H. pylori. Rabbit antiserum
against these preparations was used to detect them in fecal
antigenic extracts (FAEs) of infected patients.
Results: By immunoblotting, we were able to detect a 26 kDa
band in the positive stool samples. Anti-Olvllיs acted more
specifically, so, it was used to isolate the Helicobacter pylori
diagnostic antigens (HpDAs) from the stool. More antigens (at
least 4 antigens with the molecular weights of about 14,26,55
and 57 kDa) were isolated by affinity chromatography. But,
the 26 kDa antigen had a higher concentration and was seen in
almost all positive samples.
Conclusion: Since the 26 kDa antigen is detectable by these
two techniques in all positive samples, we are confident that
this antigen is one of the major antigens of H. pylori, which is
released into the stool and can be considered as a candidate
diagnostic antigen to be used in diagnostic kit development.
