چكيده لاتين :
transplants into the spinal subarachnoid space can alleviate
neuropathic pain behaviors. The aim of the present study was to test
the possibility that histological changes of the sciatic nerve in a
neuropathic model as well as sensory dysfunction are repaired by
adrenal medullary transplantation.
Material and Methods: Left sciatic nerve was ligated in three
groups of rats by 4 loose ligatures (eel). After one week of nerve
constriction, rats of first group were implanted with adrenal medullary
tissue (eel + adrenal medulla) and rats of the second group with
striated muscle at the level of L1-L2 (eel + muscle). The third group
received only left ligature (eel) and in the fourth group the sciatic
nerve was exposed and then muscle and skin sutured (sham).
Behavioral assessment was evaluated before surgery and 2, 4, 7, 10,
14, 21, 28, 42, and 56 days after the onset of experiment. According
to behavioral results, 4 rats in each group were anesthetized and
then the distal part of sciatic nerve were isolated and prepared for
histological quantitative investigation of nerve regeneration.
Results: The results showed that eel was accompanied with
hyperalgesia and morphological changes in the distal part of sciatic
nerve. In animals with adrenal medullary transplantation, not only
hyperalgesia was markedly reduced or even eliminated, but also the
number of myelinated fibers in the distal segment of nerve increased
to nearly normal.
Conclusions: Our findings showed that the implantation of adrenal
medullary tissue might have caused regeneration of ligated nerves
as well as alleviation of pain behavior.
