پديد آورندگان :
Mir Mohammad Sadeghi Hamid نويسنده , Yazdanian, Maryam نويسنده , Moazen Fatemeh نويسنده
چكيده لاتين :
Lipases catalyze hydrolysis and synthesis of triacylglycerols. These enzymes,
especially the microbial ones, have wide commercial and industrial usage. Bacillus
subtilis lipase has interesting properties such as small size and tolerance to basic pH;
therefore, developing techniques for its recognition and isolation is the focus of
research in many laboratories. In the present study, two factors i.e. MgCl2
concentration and annealing temperature were manipulated for the optimization of
the polymerase chain reaction (PCR) condition. As for the MgCl2 concentration, 2.5
mM produced the best results when the annealing temperature was kept at 55 oC.
Regarding the annealing temperatures, the best amplification was obtained at 63.9
oC. It can be concluded that the PCR conditions for the detection of lipase gene in
Bacillus subtilis has been optimized and can be used for the screening and isolation
of these bacaterial strains.
