چكيده لاتين :
Background: It was shown that olive leave extract has antifungal,
antibacterial and antiviral activities, The effects of
OLE on herpes simplex virus-I (HSY-I) have not been systematically
investigated yet. The aim of this study was to examine
the in vitro effect of olive leaf hydroalcoholic extract
(OLHE) on HSY-1.
Methods: Yirucidal effect and viral replication in Vera cell
line were studied in the presence of various concentrations of
OLHE applied at different time intervals using a standard
plaque assay method. The 50% cytotoxic concentration
(CCso), 50% inhibitory concentration (IC so), and therapeutic
index of OLHE were determined.
Results: OLHE showed virucidal effect on HSY-I in concentrations
> I mg/mL. The CCso of OLHE for Yero cells and ICso
were 1.75 and 0.66 mg/mL, respectively. When applied to cell
culture infected with the HSY-1, one hour earlier, OLHE
showed no antiviral activities. When applied to the cells followed
by the virus infection one hour later, or to the media
containing the virus and the combination was added to cell
culture one hour later, OLHE showed anti-HSY-I activities at
concentrations > I mg/mL.
Conclusion: OLHE has anti-HSY-I activity likely due to the
prevention of virus entry into the cells.
