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The effect of mouse embryonic fibroblast in direct differentiation of mouse embryonic stem cells

Hashemi-Tabar Mahmoud نويسنده , Javadnia Fatemeh نويسنده , Orazizadeh Mahmoud نويسنده , Sakei Ghasem نويسنده , Baazm Maryam نويسنده

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Background: Since embryonic stem (£S) cells have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, ES cells could potentially provide an unlimited cell supply for human transplantation. Objective: In order to study the differentiation of mouse embryonic stem (mES) cells, they were cultured in suspension by using ES media without Leukemia Inhibitory Factor (LIF) to induce spontaneous differentiation. Cellular morphology of differentiated derivatives was then evaluated. Materials and Methods: Undifferentiated mES from our laboratory were cultured in three different settings by using ES media containing 0.1% / 1mM trypsin/EDTA and removing LIF; in the absence of murine embryonic fibroblast (MEF) feeder cells (group 1), in the presence of MEF feeder cells with a density of 0.5