مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

Background: Recurrent abortion is a difficult medical problem happening in about 1-2% of fertile women. Most spontaneous miscarriages which happen in the first and second trimesters are caused by chromosomal abnormalities. Objective: The present study tries to find the rate of chromosomal abnormalities in couples with recurrent pregnancy loss. Materials and Methods: In total 165 couples were referred to genetic counselling clinic with a history of at least three previous abortions. In all women antibodies against toxsoplasmose, rubella and cytomegalovirus (CMV) were analysed by ELIZA. In 88 couples karyotyping was conducted by analysis of G and/or C banding. Metaphase spreads were made from phytohaemaglutinin-stimulated peripheral lymphocytes using standard cytogenetic techniques. The chromosomal status was analyzed using CytoVision Ultra ver.4.0 from Applied Imaging. The x2-test and ANOVA were used for statistical evaluation. The level of p<0.05 was considered as significance. Results: Most of the patients had 3 repeated abortions (61.2%). Cytogenetic analysis performed for 88 couples and karyotypes of 12.50/0 of them were abnormal. The majority of them had monosomy X (6.82%), followed by balanced translocation (2.27%). The number of female carries chromosomal abnormality exceeded significantly than of male. Coefficient of inbreeding in more than 50% of couples hadfifth degree of relationship (89 out of 165). Conclusion: Our results showed that 12.50/0 of the couples with missed abortion had an abnormal karyotype, with no other abnormality, Cytogenetic findings in spontaneous aborted specimens could provide valuable information for genetic counseling