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TGF-(BETA) Associated Peptide Promotes Remodeling of Healing Cutaneous Wounds in the Rat

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Background: The process of wound healing involves integrated events including inflammation, granulation tissue formation, matrix deposition and remodeling. Growth factors playa key role in the process. Among them transforming growth factor-131 (TGF-I3I) is known to accelerate tissue repair by promoting the synthesis and deposition of extracellular matrix proteins. However, persistence or overactivity of TGF-131 during the remodeling phase can potentially lead to fibrosis. The primary objective of this study was, therefore, to determine the effects of TGF-131 inactivation, by its latency associated peptide (LAP), on the cutaneous healing wounds. Methods: Excisional wounds were generated on the back of male adult rats. Wounds received TGF-131 or LAP during the post-inflammatory phase. Expression of type I collagen and asmooth muscle actin was evaluated by Western blotting. Wound maturation was further assessed by histology and immunohistochemical methods using specific antibody for proliferating cell nuclear antigen (peNA), Results: Wounds treated with TGF-131 showed a marked increase in the level of type I collagen, whereas no significant changes were observed in the wounds treated with LAP as compared to that in control. Expression of a-smooth muscle actin was markedly reduced in the wounds treated with LAP but was slightly increased in the wounds treated with TGF -131. Both neodermis and newly-formed epidermis exhibited a higher degree of maturation in the LAP-treated wounds as compared to TGF-131 treated wounds. Conclusion: Local administration of LAP seems to be beneficial to tissue remodeling. It promotes wound maturation and, may prevent fibrosis and hypertrophic scarring.

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