چكيده لاتين :
BACKGROUND: Telomerase is a reverse transcriptase enzyme that synthesizes telomeric DNA on chromosome ends.
The enzyme is important for the immortalization of cancer cells because it maintains the telomeres.
METHODS: Telomerase activity (TA) was measured by fluorescence-based telomeric repeat amplification protocol (FTRAP)
assay in prostate carcinoma and benign prostatic hyperplasia (BPH).
RESULTS: TA was present in 91.4% of70 prostate cancers, 68.8% of 16 prostatic intraepithelial neoplasia (PIN), 43.3%
of 30 BPH*, 21.4% of 14 atrophy and 20% of 15 normal samples adjacent to tumor. There was not any significant correlation
between TA, histopathological tumor stage or gleason score. In contrast to high TA in the BPH* tissue from the
cancer-bearing gland, only 6.3% of 32 BPH specimens from patients only diagnosed with BPH were telomerase activity-
positive.
CONCLUSIONS: These results indicate that TA is present in most prostate cancers. The high rate of TA in tissue adjacent
to tumor may be attributed either to early molecular alteration of cancer that was histologically unapparent, or to the
presence of occult cancer cells. Our findings suggest that the re-expression of telomerase activity could be one step in
the transformation of BPH to PIN.
