چكيده لاتين :
Background: gama-Aminobutyric acid (GABA) is considered to be the predominant inhibitory neurotransmitter in mammalian central nervous systems (CNS). There are two major classes of GABA receptors: GABAARs and GABABRs. The GABAAreceptor is derived from various subunits such as alphal-alpha 6, betal-beta 3, gammal-gamma 4, delta, epsilon, pi, and rhol-3. Intensive research has been performed to understand and establish the distribution and functions of these receptors in the CNS and peripheral tissues. The presence ofsome GABAAreceptors in spenn prompted us to explore the existence ofGABAAreceptors in rat testis and sperm.
Materials and Methods: Total cellular RNA was isolated from Wistar rat sperm and testis and reverse transcriptased to eDNA. PCR reactions were performed in a 20,u1 (microliter) volume
containing specific GABAAR subunits (forward and reverse primers) with other required materials.
Reactions were carried out using a PCR machine to investigate the existence of GABAAreceptor
subunits in rat testis and sperm. The amplification products were analyzed on 2% agarose gels
stained with ethidium bromide.
Results: Our results showed that GABA ARs composed of as, beta1, beta3, and gama1 subunits were expressed in both testis and sperm. These results indicate that, in sperm, GABAAreceptors might
have important functions.
Conclusion:
Sperm could be a novel site of GABA Aexpression . Further studies should be taken to explore the
role of these receptors on sperm acrosome reaction.
