مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

Background: Thi s study was desi gned to dev elop a new co-culture system, assess the effect of lute inizing hormone (Lll) usin g sequential media to promote de velopment and increase the qual ity of2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages, Materials and Methods: Monolayers for co-culture were prepared from ovine oviduct epitheli al cell s (OOEC) in DMEM/FI 2 medium and in-viva-fertilized 2-cell embryos were collected by flushin g from supero vulated mice . Co-culture media was treated with IOng/ml lH. For the control groups, embryos were cultured solely in G I/G2™Ver.5 drops and containing lH; and on OOEC monolayers in GlIG2™Ver.5 drops alone and containing lH as the experimental groups. Development and quality rate s were determined for all embry os daily and statistically compared. At the end of the culti vation period, differentially stained trophectoderm (TE) and inner cell mass (ICM) of expanded blasto cysts from each group were examined micro scopically. Results: The embryos cultured on an OOEC monolayer in GIIG2™Ver.5 drops treated with LH had a significantly higher developmental rate than those of the group without lH and the control groups ( p~O . 0 5 ) . The blastocysts from the lH treated co-culture, in comparison with the group without lH and the control groups, also had a significantly higher mean cell number (p~O.05). Conclusion: These findings suggest that ele vated periovulatory lH levels may promote preimplantation embryo development. These results have important implications for assi sted reproductive technologies in which co-cultures are used to improve pregnancy rates. OOEe cell co- culture system treated by lH could improve in vitro preimplantation embryo development both in terms of quality (increasing blastocyst cellularity ) and developmental rate.