چكيده لاتين :
The abilities of Silybum marianum cell culture to biotransform benzaldehyde
to benzyl alcohol in three culture systems were compared. Callus cultures of
Silybum marianum were established from seedlings, and healthy suspensions and
immobilized cultures grown on the Murashige and Skoog medium. S. marianum cells
were immobilized in both agar beads and fiber cotton matrixes. Benzaldehyde was
fed to S. marianum cell suspension and immobilized cultures. Biotransformation
reactions were detected over 24 hours of incubation. The cultures then were
extracted with dichloromethane and the extracts were subjected to GC and GC-MS
analysis. The S. marianum culture systems exhibit different conversion rates in the
reduction of benzaldehyde. Immobilization seemed to have an effect on the secondary
metabolism, the cells immobilized in fiber cotton matrix were more efficient at
performing the reduction process than both the freely suspended and agar immobilized
cells. The ability of cultured plant cells to biotransform substrate appears to be
dependent on the type of the culture system.
