چكيده لاتين :
Angiocardiography is an X-ray examination of the blood vessels or chambers of the
heart. Cardiologists and staff members applying this procedure are exposed to high levels of
scattered radiation. In our previous study the incidence of unstable chromosomal aberrations
and cytokinesis-blocked micronuclei were found to be significantly higher in exposed
individuals than the age and sex matched controls.
In the present study we assessed cytokine production by peripheral blood mononuclear
cells of the above cases and the percentage of Treg cells. According to filrn dosimeter
analysis, personnels received 0.25-15 mSv during the previous year (average of 3 mSv 1י1)י
Isolated PBMCs from the test and control groups were stimulated with Phorbol Myristate
Acetatel Ionomycin (PMA/I). Cytokine production was measured in the supernatants of
cultured lymphocytes. The percentage ofTreg cells was studied by flow cytometry.
The production of IL-lO and IL-5 was sibrnificantly down-regulated in the test group
compared to the control group. In contrast, IL-12 was up-regulated. Yet, no statistically
significant difference was found for IFN- y between two groups. In addition, we found higher
percentage of CD4+CD25+bright Treg cells in the study group compared to the controls.
Taken together, it was shown that low doses of scattered X-rays could skew cytokine
profile of peripheral blood mononuclear cells in favour of inflammatory response causing
the increase of Treg cells.
