عنوان به زبان ديگر :
Detection, cloning, molecular characterization and phylogenic analyses of a new primate T-Cell lymphotropic virus type I in olive baboon
پديد آورندگان :
Azadmanesh Kayhan نويسنده , Roohvand Farzin نويسنده , Amini Safieh نويسنده , Kazanji Mirdad نويسنده
چكيده لاتين :
Infection with Human T-cell Lymphotropic Virus Type I
(HTLV-I) is a global health problem, affecting 10 to 20
million people around the world, including north-east of
Iran. It has been recognized to be the etiologic agent of
adult T-cell Leukemia and HTLV-I-associated
Myelopathy. In both cases, the HTLV-I transactivator
protein (Tax), plays crucial role. Monkeys are suitable
host for a related virus called STLV, which together
with HTLV are called Primate T-cell Lymphotropic
Viruses (PTLVs). Primates are the only known hosts, in
addition to human, to be able to develop malignant
changes in the natural course of infection with PTLV-I
and therefore, could be a valuable animal model for
studies on this virus. In the present study, we report
PCR-based detection and cloning of 1.8 kb pX region
of a new PTLV in olive baboon (Papio anubis).
Sequence alignments and phylogenic studies on
nucleotide sequence of this region and amino acids of
conceptually translated Tax protein showed that monkeys
are infected with a PTLV much closer to HTLV-I
sub-types a and b, rather than STLV-I. Moreover,
analyses of its Tax protein suggest that it might have
the same function as HTLV-I Tax proteins. Results of
our study indicate the possibility of exploiting these
baboons as an animal model of choice for evaluating a
tax-based DNA vaccine to decrease the viral load of
HTLV-I in carriers , in order to prevent the outcomes,
as well as they may be utilized for other HTLV-I physiopathologic
or therapeutic studies.
