پديد آورندگان :
Kulandaisamy Venil Chidambaram نويسنده , Nanthakumar Kuppanan نويسنده , Karthikeyan Kannan نويسنده , Lakshmanaperumalsamy Perumalsamy نويسنده
چكيده لاتين :
This paper describes optimization method that combines
the Plackett-Burman design, a factorial design
and the response surface method, which were used to
optimize the medium for the production of L-asparaginase
by Serratia marcescens SB08. Four medium factors,
from out of 11 medium factors, were screened by
Plackett-Burman design experiments and subsequent
optimization process to find out the optimum values of
the selected parameters using central composite
design was performed. Sucrose, peptone, KH2PO4
and incubation time were found to be the best medium
factors for the optimization of L-asparaginase production
and central composite design experiments indicated
the optimal concentrations of sucrose 12.50 g/l,
peptone 4.5 g/l, KH2PO4 4.0 g/l and incubation time
51h. The combined optimization method described
here is the effective for screening medium factors as
well as determining their optimum levels for the production
of L-asparaginase by Serratia marcescens
SB08.
