چكيده لاتين :
Micropropagated plantlets derived from three different
grape rootstock genotypes namely, Dogridge
(Vitis champini), SO4 (V. berlandieri×V. rupestris) and
ARI-H-144 (V. vinifera×V. labrusca) were subjected to
randomly amplified polymorphic DNA (RAPD) and
inter simple sequence repeats (ISSR) analyses in
order to evaluate their genetic stability and/or detect
likely existing variations among in vitro derived
plantlets. A dozen RAPD (10-mer) and ten ISSR (dinucleotide
contained repeats) primers were used for
PCR and reproducible band profiles were obtained.
The 84 and 81 distinct and scorable band classes (a
total of 1,914 and 1,980 scorable bands) with an
average of 7.0 and 8.1 bands per primer were
obtained by RAPD and ISSR, respectively. Although
higher numbers of bands were obtained by ISSR
rather than RAPD, but none of the primers showed
polymorphism among micropropagated plantlets and
their respective mother plants. The profiles generated
based on the two marker systems were found to be
highly uniform and monomorphic. Cluster analysis
further confirmed genetic stability of micropropagated
plantlets. Jaccard’s similarity coefficients obtained for
both markers in mother plants and their in vitro regenerants
were estimated to be 1.00 but three sets of
genotypes were grouped into two major clusters with
similarity coefficients of 0.53 (RAPD) and 0.63
(ISSR). The molecular analyses precisely proved the
production of genetically stable grape plantlets and
certified the application of micropropagation protocol
to be developed on a commercial scale.
