عنوان به زبان ديگر :
Evaluation of stability of chitinase gene in transgenic offspring of cotton (Gossypium hirsutum)
پديد آورندگان :
Tohidfar Masoud نويسنده , Rassouli Hassan نويسنده , Haghnazari Ali نويسنده , Ghareyazie Behzad نويسنده , Najafi Javad نويسنده
چكيده لاتين :
Cotton cultivar Coker has been already transformed
with recombinant pBI121-chi via Agrobacterium tumefaciens.
The T-DNA region of pBI121-chi carries the
chitinase (chi ) gene from bean and is under the control
of the CaMV35S promoter. T1 and T2 progenies of
transgenic cotton containing the chi gene were used in
this study. Polymerase chain reaction (PCR), Southern
and Western blotting data confirmed integration and
expression of the chi gene in the T1 and T2 progenies.
The growth of Verticillium dahliae was singnificantly
inhibited in an in vitro bioassay for which 100 ىg of
crude leaf protein extract derived from the T1 plants
was used. The 850-bp expected chi fragment was
amplified for 77 transgenic plants from 128 T1 and T2
progenies, and 75 transgenic plants showed both chi
and nptII bands. T0 conduct bioassay, cotton seedlings
were infected with the spore suspension (106
spores/ml), in a greenhouse. Fifty-five percent of the
transgenic plants were able to restrict V. dahliae growth
and symptoms. There were no distinguishable differences
in the phenotypic appearance of transgenic
plants compared to non-transgenics. These results
showed that transgenic cotton expressing a bean chitinase
exhibited enhanced resistance against V. dahliae
in greenhouse and in-vitro assay as compared to the
non-transgenic plants.
