مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

The aim of the present study was to characterize some Iranian wild species of Valerianaceae with respect to their contents of valepotriates. Identification of these compounds which are known as reliable markers in Valerianaceae family was achieved using TLC and UV-spectrophotometry methods. Valepotriates makes substantial contribution to the sedative effect of valerian. Separation of these compounds was performed by TLC using a ternary mobile phase of toluene-ethyl acetate - methyl ethyl ketone (80: 15:5 v/v) on silica gel HF254+366 plates. Separated bands were observed in ultraviolet light at 254 nm. For bands revelation, 2, 4dinitrophenylhydrazine (DNPH) solution spray was used at visible light. For the quantitative determination of valepotriates a standardized product (Valmane®) was used to plot two calibration curves. One calibration curve was drawn in the concentration range of 5-156 mg L-1 for determination ofvaltrate + acevaltrate amounts. The second one was linear in the range of 5-52 mg L-I for didrovaltrate quantification. The qualitative and quantitative determinations were carried out in all organs of Valeriana sisymbriifolia Vahl, Valeriana alliariifolia Adams and Centranthus longiflorus Stev. (Iranian wild plant species) and commercial Valeriana officinalis L. sample. Results showed that didrovaltrate amounts (dry weight basis) ranged from 0.13°~ in inflorescence of V. alliariifolia to 3.51o~ in leaf of C. longiflorus. Also acevaltrate + valtrate contents varied from minimum 0.06°~ in stem of V. sisymbriifolia to maximum 10.85% in root of V. alliariifolia. Valepotriates contents in these species were compared to those of commercial Valeriana officinalis L. root and rhizome samples (didrovaltrate 1.99-3.23°~, valtrate + acevaltrate 5.1-9.26%).