چكيده لاتين :
In the current investigation, primary porcine brain capillary endothelial (PBCE) cells were isolated and used as an in vitro blood-brain barrier model. The bioelectrical and permeability properties were assessed in
PBCE cells treated with Astrocytes Conditioned Medium (ACM), hydrocortisone and cAMP elevators. The primary PBCE cells cultured with ACM plus cAMP elevators provided significantly higher trans-endothelial electrical resistance (~700 omega cm) in comparison with those cells cultured with ACM (~400 omega cm) or untreated control (~200 omega cm), while PBCE cells cultured with ACM plus hydrocortisone (550 nM) resulted in ~550 omegacm. The bioelectrical properties were further validated by means of the permeability coefficients of transcellular and paracellular markers with results of ~20 and ~5 (x 10^-6 ern sec^-I) for propranolol and sucrose, respectively in PBCE cells treated with ACM and cAMP elevators. Upon the obtained bioelectrical and permeability results, PBCE cells cultured with astrocytes conditioned medium and cAMP elevators can be proposed as a high-throughput in vitro model for drug screening and brain targeting.
