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Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat

Marzban Mohsen نويسنده , Bakhtiary Mehrdad نويسنده , Mehdlzadeh Mehdi نويسنده , Taghi Joghataei Mohammad نويسنده , KHOEI SAMIDEH نويسنده , Pirhajati Mahabad Vahid نويسنده , Ebrahimi Asghgar نويسنده

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Introduction and Objective: This study was designed to investigate the effects of granulocyte colony-stimulating factor (G-CSF) administration in rats for 6 weeks after traumatic brain injury (TBI). Materials and Methods: Adult male Wistar rats (n = 30) were injured with controlled cortical impact device and divided into four groups. The treatment groups (n = 10 each) were injected subcutaneously with recombinant human G-CSF. Vehicle group (n=10) received phosphate buffered saline (PBS) and only Brdu intraperitoneally. Bromodeoxyuridine (BrdU) was used for mitotic labeling. Experimental rats were injected intraperitoneally with BrdU. Rats were killed at 6th week after traumatic brain injury. Neurological functional evaluation of animals was performed before and after injury using neurological severity scores (NSS). Animals were sacrificed 42 days after TBI and brain sections were stained using Brdu immunohistochemistry. Results: Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (p