چكيده لاتين :
Micron-sized polystyrene (PS) seed particles were prepared by dispersion polymerization of styrene in ethanol-water media. Seeded copolymerization ofN-isopropyl acrylamide (NIPAM), methacrylic acid (MAA) and N,Nי -methylene-bis-acrylamide (MBAAm) was then carried out in presence of PS seed particles. The produced PS/P(NIPAM-MAA-MBAAm) composite polymer particles exhibited volume phase transition to small changes in temperature and pH, respectively. Specific activities of trypsin aqueous solution (free trypsin) and trypsin in presence of PS seed and composite polymer particles were measured under identical conditions. Relative study suggested that trypsin adsorbed on PS/P(NIPAM-MAA-MBAAm) composite polymer particles retained their native conformation.
