پديد آورندگان :
Ghanbari M. نويسنده , REzaei M. نويسنده , Soltani M. نويسنده , Shah-Hosseini Gh. نويسنده
چكيده لاتين :
Bacteriocins are proteinaceous antibacterial compounds that exhibit bactericidal activity against species
closely related to the producer strain. The aim of this research was to investigate the production of
bacteriocin by Bacillus spp. isolated from intestinal bacterial flora of the Caspian Frisian Roach (Rutilus frisii
kutum). A bacteriocin produced by the bacterium Bacillus cereus strain RF 140 was identified. The
antimicrobial activity started at the exponential growth phase and maximum activity was at the stationary
growth phase. A crude bacteriocin obtained from culture supernatant fluid was inhibitory to indicator strains,
including Listeria monocytogenes, Clostridium perfringens, and several species of Bacillus. Bacteriocin was
stable at 80°C, but the activity decreased and was lost when the temperature reached 100 and 121°C,
respectively. It was resistant to the proteolytic action of papain, catalase and amylase, but sensitive to
proteinase K, pronase E and trypsin. Maximum bacteriocin activity was observed in the pH 4-9. This study
indicates the importance of the bacteriocin produced by B. cereus strain RF 140 against food-borne
pathogenic microorganisms such as L. monocytogenes and C. perfringens, and presents a potential for use as
a biopreservative in food.
