چكيده لاتين :
Shrimp waste is the most important chitin source for commercial use. In this study chitin and chitosan
were extracted from Penaeus semisulcatus waste collected from a shrimp processing landing center
situated at Persian Gulf in south of Iran by chemical and microbial methods. Chitin and chitosan were
extracted by alkali-acid treatment and the yields were 510 and 410mg/g, respectively. Demineralization
is an important step in the chitin purification process from shrimp waste. Chemical extraction method
included the use of NaOH solution and acetic acid. In microbial extraction, organic acids (lactic acid)
produced by probiotic bacteria was used to demineralize microbial deproteinized shrimp shells. The study
showed that the effectiveness of using lactic acid bacteria especially added Fe (NO3)3 as extra nitrogen
source for demineralization of shrimp shells than chemical method (1750 against 810mg/g). Chitin and
chitosan extracted from shrimp waste by chemical and microbial methods was crystalline powder, nonharmful
and odorless, white and off-white, respectively. The moisture content was calculated as 63.8%.
The amount of Ca, Fe, Cu and Mn present in the shells was 168, 35.58, 38.28 and 6.72mg/L, obtained by
atomic absorption spectroscopy, respectively. The amount of calcium in the shells was 25 times higher
than manganese. The results suggested Lactobacillus plantarum (PTTC 1058) is an attractive source of
recovery for chitin and chitosan.
