چكيده لاتين :
In industrial hydrolysis processes the lignocellulose-degrading enzymes may have a potential application as an
alternative to chemical treatments because they produce high specificity, mild reaction conditions, no toxic chemical
hydrolysis inhibitors, and no loss of substrate due to chemical modifications. The amounts of reducing sugars released
from the degradation of wheat straw by the action of endoxylanase, peroxidase, Beta-xylosidase, and alpha-L-arabinofuranosidase,
which were produced by T. fusca BD25, were equal to 3.0%, 0.2%, 0.3%, and 0.2% hydrolysis (as
xylose equivalents) of the substrate used, respectively, after 10 h of incubation. In the same conditions, the hydrolysis rate
of endoglucanase, which was a commercial preparation from Trichoderma viride was 4.9%. However, in order, addition
of peroxidase, endoglucanase, Beta-xylosidase, and alpha-Larabinofuranosidase preparations to the endoxylanase preparation
significantly enhanced the reducing sugar yields (1.33-, 1.52-, 1.61-, and 1.71-fold, respectively) and the hydrolysis of
wheat straw (4.3%, 12.3%, 13.5%, and 14.7%, respectively). The concentrations of phenolic-compounds as ligninequivalents
released from wheat straw by the actions of lignocellulose-degrading enzyme preparations either acting alone
or in combinations ranged from 2.5 to 129.3 mu g mL-1 (as p-coumaric acid equivalents). The combination of purified
lignocellulose-degrading enzymes significantly increased the degradation of straw 1.33- to 1.71-fold. The results highlight
the role of lignocellulose-degrading enzymes in the degradation of wheat straw and suggest that the use of enzyme
cocktails may significantly improve the hydrolysis of wheat straw in industrial processes.
