چكيده لاتين :
A protocol has been developed for the direct induction of adventitious shoots from leaf explants of in vitro
raised Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. The Murashige and Skoog (MS) media
supplemented with N6–benzyladenine (BA), kinetin (KIN), and thidiazuron (TDZ) were used in regeneration
experiments. BA at 0.5 mg L-1 concentration was chosen as the optimal treatment because it yielded the maximal number
of adventitious shoots with the best quality (6.70 ± 1.05 shoots/explant). The highest mean of maximum shoot length was
observed in MS media containing 0.2 mg L-1 BA (4.30 ± 0.48 cm). Regenerated shoots rooted best on ½MS medium
containing 0.5 mg L-1 indole-3-butyric acid (IBA). Flower buds also occurred during rooting. For this study, the existence
of auxins in the medium was essential for in vitro flower bud induction. IBA was found to be more efficient than indole-
3-acetic acid (IAA) in the induction of flower buds of A. xylopoda. The maximum flowering percentage was obtained
when the shoots were cultured on ½MS medium containing 1.0 mg L-1 IBA. Flowers produced in vitro were
morphologically normal and yellow.
