23473

Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçükِdük using conventional and in vitro techniques

327

333

7

This report deals with the successful clonal propagation of endangered T. turcica using rhizome cuttings and epicotyl explants. Rhizome cuttings were treated with ل-naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA) before planting for vegetative multiplication. Rhizome cuttings pretreated with NAA (10 mg/L) were both rooted and sprouted (66.6%) after 100 days. Application ofNAA induced callus and adventitious root formationin epicotyl explants and 6-benzyladenine (BA) induced production of microshoots. Low levels of NAA (0.5-1 mu M) together with BApromoted shoot initiation and development. The highest regeneration rate (86.6%), with a mean number of shoots (3.05) and a mean length of shoots (2.3 cm) per epicotyl, was achieved at 10mu M BA and 0.5 mu M NAA. About 83% of in vitro regenerated shoots rooted on a ½ Murashige and Skoog (MS) medium supplemented with 0.3 mu M NAA. In vitro plantlets were morphologically normal and a uniform chromosome complement of 2n = 18 was detected in root tips. The study demonstrated that both conventional and in vitro techniques could be useful for large scale multiplication and propagation of this endangered plant species.

1207545