Prevalence, Antibiotic resistance of Staphylococcus aureus, CNS and determination of MRSA, MRCNS strains in clinical samples

A total of 113 strains of S. aureus and CNS were isolated from clinical samples from three hospitals in Damascus city between 6/2013 – 8/2014, and were identified by biochemical and molecular tests. The percentage of S. aureus was (65.48%), followed S. epidermidis (25.66%) then S. saprophyticus (4.42%), so the prevalence of S. aureus was major in (pus, abscess) which was 28.37%, while the prevalence of S. epidermidis was more in CSF which was (31.03%) The molecular methods and antimicrobial resistance were studied in 55 strains of collection of samples (40 S. aureus and 15 CNS), where detected 16SrRNA, gap, and nuc genes for adjust the identification. Antibiotic resistance tests examined for 8 different antibiotics as follows: AMC (65%), GN (30%), CRO (62.5%), PE (7.5%), OFX (7.5%), AM (70%), VA (2.5%) and AX (55%). The percentage of presence of MRSA strains was 15% and MRCNS was 6.66%.