Quality enhancement of cryopreserved spermatozoa of sutchi catfish(Pangasianodon hypophthalmus) with honey addition

Sutchi Catfish is one of the important fish commodities in Indonesia. Unfortunately, its seasonal spawning pattern causeslimited supply. Cryopreservation is a solution to solve limited supply since it can store the spermatozoa in low temperature sothat physiological, biological and morphological functions still remain. Improving the quality of cryopreservation is importantto increase the success of Sutchi Catfish aquaculture. Adding honey in cryopreservation process is expected to increase thequality of spermatozoa since it contains with sugars as a source of spermatozoa’s energy. This study tried to compare theeffectivity of honey in cryopreservation process with no addition. The treatments used in this study were T1 (0% honey), T2(0.2% of honey), T3 (0.4% of honey), T4 (0.6% of honey) and T5 (0.8% of honey). 30 days after stored, the spermatozoa werechecked their motility, viability, abnormality, fertility and hatching rate. This study showed that honey addition could increasethe motility significantly (P 0.01) to 23.14% better than control. The viability increased significantly (P 0.01) to 23.17%better than control. The abnormality test did not show significant difference between honey addition and control although theabnormality value in control was the highest (10.75%). The fertilization rate increased significantly (P 0.01) to 28.85% betterthan control. The hatching rate increased significantly (P 0.01) to 29.78% better than control. The success of all test indicatedthat the addition of honey in cryopreservation process of spermatozoa could be performed on Sutchi Catfish to increase itsproduction even though the limited spawning pattern.