Histological and fluorescent microscope studies for evaluation carbon accumulation in trachea and bronchi of birds in polluted area in Wasit province

The aim of this study was to detect the accumulation of carbon and determination its appearance in different areas ofgroups of ducks. Using special dyes to detect deposits of carbon particles. Also, using AO/ EB stains to detect early and late(progress) apoptosis that occurred due to the precipitated of carbon in both areas (Al-ahdeb oil field and brick factories areas)but late apoptosis occurred in bronchi of brick factories area more than oil field area. The histological examination of tracheashowed no any indicator of accumulation of carbon in three different areas, whereas in bronchi showed the presence of carbonin polluted areas (Al-ahdeb oil field and brick factories areas) in different amounts.