دراسة مرضية للخمج التجريبي بجراثيم المكورات المعوية البرازية في طيور السمان

This study was conducted to isolate and classify Enterococcus faecalis bacteria from the small intestine and caecum of quail birds and grow them in differential and selective culture media and determine the concentration of Enterococcus faecalis suspension for experimental infection and pathological follow-up. Quail birds were divided randomly into four groups, the first group was considered a control group and the following groups were injected with 0.5 ml of the bacterial suspension inside the chute, where the second group was injected with a concentration of 1X108CFU and the third group was injected with a concentration of 1X109CFU, and the fourth group was injected with a concentration of 1X1010CFU. Clinical signs and lesions were followed up. Pathology after performing the anatomy of quail during periods 3, 7, 14 and 21 days after infection of the heart, liver and kidneys. The results showed the growth of Enterococcus faecalis bacteria after bacterial isolation, as well as the presence of macroscopic lesions represented by opacity of the pericardium, hypertrophy of the heart and liver infarction. As for histologically, the lesions included the beginning of endocarditis, the appearance of severe fatty change, localization of recent clots and severe necrosis of hepatocytes. The kidney also showed cellular swelling Renal tubule epithelium and programmed cell death (Apoptosis) on the third day of infection and the tissue lesions were generally more severe at days 3 and 7 of infection. We conclude from this study that quail birds have a strong defense and immune mechanism despite the appearance of pathological changes at experimental infection with high concentrations of bacterial suspension that cause death in other animals such as mice and rats, in addition to that Enterococcus faecalis has the ability to stimulate programmed cell death. .