Role Of Pomegranate Seed Oil (PSO) Against Hepatotoxicity Induced By Sodium Fluoride In Adult Female Rats (Part II)

This work was designed to evaluate the hepatoprotective effects of pomegranate seed oil (PSO) against toxicity induced by sodium fluoride (SF) in adult female rats. Twenty female rats were divided into four equal group and treated daily for 40 days as follows: Group C administered tap water and served as control , group T1 : received sodium fluoride (120mg/liter) in drinking tap water, group T2: received both sodium fluoride (120mg/liter) in drinking water and administered orally pometone (PSO) 30mg/kg B.W. and group T3 : administered PSO 30mg/kg B.W. orally. Fasting blood samples were collected at 0, 20 and 40 days to estimation of some biochemical parameters and oxidative stress biomarkers . In addition, sections from liver were taken at the end of the experiment for histopathological study. The results revealed that SF treated group caused a significant increase in serum aminotransferases (ALT and AST) activity, total cholesterol ,total bilirubin and peroxynitrite radical concentrations, while GSH concentration was a significantly decrease. PSO caused an alleviation to the hepatic dysfunction induced by sodium fluoride manifested through significant elevation of GSH concentration, in addition, a significant reduction in serum transaminases activity, total cholesterol, peroxynitrite radical and total bilirubin concentrations. In contrast, administration of PSO (group T3) restored almost most of these parameters to near or below to normal levels. Furthermore histopathological examination of liver tissues of group T1 manifested aggregation of mononucleated cells (MNCs), proliferation of hepatocyte, cytoplasmic fat droplet and granulomatous lesion consists of aggregation of macrophage and lymphocyte. All these alteration in liver histology were modified by treatment of rats with PSO. No clear pathological lesion was reported in group T3 received PSO. On conclusion, this study documented the beneficial effect of PSO against the deleterious effects of SF on liver functions of adult female rats.