دراسة التاثير التراكمي للميتوتركسات Methotrexate في تكون النطاف عند ذكور الفئران البيضاء وامكانية البراء

Intramuscular accumulative injection of Methotrexate was delivered to 40 individuals of 16-week old adult mice with a 32 g weight average. The given dose was 15 mg/kg and delivered twice a week. A decrease in the survival rate was noticed as a result after the second injection, and this decrease in the survival rate continued to reach 10% after the fourth injection .This indicates that this 15 mg/kg dose has a lethal effect when it is used in an accumulative pattern. The same previous experiment was repeated using a 10mg/kg dose and delivered to 45 adult mice, this delivery covered 8 weeks time in the experiment. All of the tested animals survived the given dose. This indicates that this accumulative dose is biologically tolerated by the tested mice. Whereas the spermatogenesis indicators were rapidly decreased in the treated animals which affected the fertility abilities in the male mice; the testes weight decreased gradually compared to the control ones from 170 ± 7 mg/test to 135 ±21 mg/test, and this was accompanied by a noticed reduction in the sperms count from 106 ×18.25± 2.02 sperms/test to 106 × 4.76 ±1.28 sperms/test. It is important to mention that spermatogenesis indicators (fertility) increased gradually after the injection was stopped, and returned to its normal levels after two months of stopping. This indicates that the recovery from Methotrexate effects is possible after the injection was stopped.