Role of spermatozoa in pH stability of caudal epididymis environment

50 ram testicles where been collected directly after slaughtering, preserved in cool box (8-10°C) and transferred to the andrological lab at the Veterinary Medicine Collage / University of Baghdad. Samples were washed thoroughly with distal water stripped from surrounding tissues. Epididymis were separated from the entire testicles, caudae were cut out and injected with warm normal saline then sliced, pH was collected daily (4-5 days). Result showed that: as the preservative periods proceed, the pH of caudal secretions is elevated as (6.22, 6.35, 6.45, 6.65, 6.66, 6.7, 6.77) at (24, 36, 48, 60, 72, 84, 96 hrs) while total motility percentage are (90, 85, 84.5, 77, 75, 75, 70%). Cold preservation after slaughtering showed decreased motility as preservation proceed as well as total alive spermatozoa decreased. In conclusion caudal secretion turned to be more acidic if cold preservation time is prolonged and total alive spermatozoa number decreased while dead one increased.