Sero-Diagnosis of Anti-Fasciola hepatica IgG-Antibodies in Blood and Milk of Cattle, Goats and Sheep by Using an Indirect-ELISA

The present study was aimed to evaluate the prevalence of anti- F. hepatica IgG-antibodies in blood and milk of cattle, goats and sheep by using an indirect-ELISA. The study was performed in different areas in Al-Qadisiyah province, during a period of April to September / 2016, and involved totally 388 milking animals; 113 cattle, 130 goats and 145 sheep. Overall results reported that 34.54% and 23.71% of blood and milk samples, respectively, were seropositives for anti- F. hepatica antibodies. Among study’s animals, prevalence of seropositive in blood and milk, respectively, was 46.02% and 32.74% in cattle, 26.15% and 14.62% in goats, and 33.1%and 24.83% in sheep. Significant differences were detected between positive results of blood and milk as well as between study’s animals (P 0.05). In regarding to intensity of infection, the mild infections (52.99% and 60.87%) were more appearance than moderates (33.58% and 29.35%) and severe infections (13.43% and 9.78%) in blood and milk, respectively. In cattle, it’s noted that the moderate infections were appeared, relatively, more obvious; while in sheep and goat, mild infections reported a high incidence than other levels of infections.