تاثير المستخلصات المائية الباردة والمغلية لجذور عرق السوس Licorice في تثبيط الاثر السمي والتطفيري لعقار الميثوتركسيت في الفئران البيض Mus musculus

The inhibitory efficiency of licorice Glycyrrhiza glabra to the toxic and mutagenic effects of methotrexate drug in mice [ Mus musculus ] was studied using short term assay as cytogenetic analysis such as mitotic index of bone marrow and germ cells , chromosomal aberrations , micronucleus test and sperm head abnormalities assays . The potential genotoxic and mutagenic activity of licorice root powder at doses 150 , 300 , 450 and 600 mg/kg body weight and the aquatic extract of licorice root (cold and boiled) at doses 100 , 200 , 300 and 400 mg/kg B. wt were investigated by using the above parameters .Simultaneously antimutation effect of powder and aquatic extract of licorice root against MTX ( at dose 3.25 mg/kg B. wt ) effects [ before and after exposure ] were tested . The results revealed the following :- All concentration of powder and aquatic extracts of licorice root at tested doses are not toxic and non mutagenic , the high inhibitory effects of methotrexate drug to cell division , induction of chromosomal aberration , micronucleus formation and sperm head abnormalities and the high efficiency of all concentration of powder and aquatic extracts of licorice root against the toxicity and mutagenicity of methotrexate when it used before or after the drug.