Molecular detection of some virulence genes of Staphylococussaprophyticus isolated from women with cystitis cases

The study included isolation and identification of uropathogenic Staphylococcus saprophyticuswhich responsible for cystitis occurrence in young women. A total of 140 mid stream urinesample were taken from women suffering from urinary tract infection that attended to the Hillageneral teaching hospital during a period from November 2014 to February 2015 . The patientsage ranged from 16 to 55 years. Only 14 isolates were identified as Staphylococcussaprophyticus which have been diagnosed by using selective medium, biochemical tests and byusing molecular assays. Molecular detection of Ubiquitous uro-adherence factor A was done byusing specific PCR primer . This marker was shown to be present in all isolates of this bacterium,while the molecular detection of the capsular polysaccharides gene capD has shown that onlyone isolate (7%) has this gene and gave positive result for this primer. Moreover, Autolysinadhesinsurface protein Aas was also detected in S.saprophyticus . It was shown that all isolates14(100%) gave positive result for this gene.The collagen binding protein gene sdrI was alsoinvestigated in this bacteria and this study has shown that only one isolate possess this gene.Inaddition to that , the surface associated protein gene of S.saprophyticus Ssp was detected in thisstudy by using specific primer in 12(85%) of isolates , this gene is responsible for lipaseencoding .