Studies on some Metabolites, Ionic and Hormonal Composition in Ovarian Follicular Fluid and Blood Serum in relation to size of the Follicle in Iraqi Buffaloes

The objective of this study was to determine the biochemical composition of follicular fluid obtained from different sized follicles and it s relationship with that of blood serum in Iraqi buffaloes. Ovaries of adult non-pregnancy and healthy buffaloes were collected from each buffalo at the time of slaughter. Follicular fluid was aspirated from small (3-10mm) and large (11-20mm) follicles and also serum separated and both stored at -20ºC for further analysis. The follicular fluid and blood serum sample were analyzed using commercial kits. The results showed that thefollicular total protein concentration difference between small and large follicles was nonsignificant. The follicular glucose concentration was significantly increased (p 0.05) from smallto large follicles. The follicular fluid concentration of total cholesterol and triglycerides, were significantly decreased (p 0.05) from small to large follicles. The blood serum concentrations ofthe total protein, glucose and total cholesterol were significantly higher (p 0.05) than follicular fluid in the small and large follicles, while blood serum triglycerides concentration wassignificantly lower (p 0.05) than follicular fluid in both classes of follicles. The follicular fluid calcium and sodium concentration were significantly increased with enlargement of follicular size. The follicular fluid potassium concentration of small follicles was significantly decreased than in large follicles, while no difference were found in concentration of magnesium between small and large follicles. The blood serum calcium, sodium and magnesium concentrations weresignificantly higher in comparison within follicular fluid, while the blood serum potassiumconcentration was significantly lower than follicular fluid in small and large follicles. Thefollicular fluid estradiol and progesterone concentrations were significantly increased with increased of follicular size while the blood serum estradiol and progesterone concentrations were significantly lower than follicular fluid in small and large follicles.