The roles of Glutathione and Ascorbic Acid in Na-detoxification in terms of rooting response of Mung bean Cuttings

The roles of glutathione , ascorbic acid, and their interaction in Na-detoxification removing in terms of adventitious root formation (ART) in mung bean cutting has been studied. The effect of different concentrations of the anti-oxidant GSH ranged between (10-^3-10^-11M) and ASA (100-600 ppm), the optimum concentration of GSH and ASA were (10^-5 M and 100 ppm) respectively. The toxic concentration of NaCl was determined by using concentrations ranging from 25-300 mM. The toxic concentration was (50mM).Combination with 1: 1 ratio (V:V) for both antioxidants ASA and GSH that developed the optimal rooting response, (19.75) roots per cutting was obtained by the combination of (100: 10^-5) ASA: GSH. Detoxification of sodium chloride stress was occurred completely by supplying combination prior to toxic–NaCl treatment (pretreatment and after toxic NaCl treatment (post treatment) compared to their supply simultaneously with toxic NaCl.